Immunoprecipitation (IP) 프로토콜 / IP 프로토콜 / Imunopreciptiation protocol / IP protocol

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​IP assay는 단백질을 다루는 실험이므로 모든 step에서 온도를 낮게 유지하는 것이 좋다. 왜냐하면 단백질은 고온에서 3차 구조가 변성되기 때문에 단백질의 결합에 영향을 줄 수 있기 때문이다. Immunoprecipitation protocol Preparing the lysates Pre-clearing the lysates (optional) Immunoprecipitation Binding A/G agarose bead Continue reading Immunoprecipitation (IP) 프로토콜 / IP 프로토콜 / Imunopreciptiation protocol / IP protocol→

Immunoprecipitation 원리 / Immunoprecipitation principle / IP 원리 / IP principle / 면역침강법 원리

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Immunoprecipitation 원리 / Immunoprecipitation principle / IP 원리 / IP principle / 면역침강법 원리

Immunoprecipitation 원리인 항원 항체 반응을 이용하여 단백질 간의 상호 결합을 (protein-protein interaction) 확인하는 실험 방법이다. 각 단백질들의 결합은 생명 활동을 조절하는데 있어서 아주 중요한 역할을 담당한다.단백질 간의 결합을 찾아내고 확인하는 것은 생체 내 여러 기작을 조절하는 메커니즘을 밝혀내는데 매우 중요하다. Continue reading Immunoprecipitation 원리 / Immunoprecipitation principle / IP 원리 / IP principle / 면역침강법 원리→

Life is not about for the waiting storm to pass

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Life is not about for the waiting storm to pass

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aliquam mattis nibh et tristique mollis. Interdum et malesuada fames ac ante ipsum primis in faucibus. Duis eu nunc vel felis convallis venenatis. Etiam maximus placerat arcu, et malesuada diam laoreet at. Continue reading Life is not about for the waiting storm to pass→ Continue reading Life is not about for the waiting storm to pass→

Life is not a long journey,it’s a joyful journey

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Life is not a long journey,it's a joyful journey

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aliquam mattis nibh et tristique mollis. Interdum et malesuada fames ac ante ipsum primis in faucibus. Duis eu nunc vel felis convallis venenatis. Etiam maximus placerat arcu, et malesuada diam laoreet at. Continue reading Life is not a long journey,it’s a joyful journey→ Continue reading Life is not a long journey,it’s a joyful journey→

Life is really Simple, But We make it complicated

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Life is really Simple, But We make it complicated

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aliquam mattis nibh et tristique mollis. Interdum et malesuada fames ac ante ipsum primis in faucibus. Duis eu nunc vel felis convallis venenatis. Etiam maximus placerat arcu, et malesuada diam laoreet at. Continue reading Life is really Simple, But We make it complicated→ Continue reading Life is really Simple, But We make it complicated→

If you love your life, life will love you back

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If you love your life, life will love you back

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aliquam mattis nibh et tristique mollis. Interdum et malesuada fames ac ante ipsum primis in faucibus. Duis eu nunc vel felis convallis venenatis. Etiam maximus placerat arcu, et malesuada diam laoreet at. Continue reading If you love your life, life will love you back→ Continue reading If you love your life, life will love you back→